ANSI/ASSE Z9.14-2014


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Committee Title: Z9 Health and Safety Standards for Ventilation Systems Accredited Standards Committee

Standard Title: Testing and Performance-Verification Methodologies for Ventilation Systems for Biosafety Level 3 (BSL-3) and Animal Biosafety Level 3 (ABSL-3) Facilities

Scope: Methodologies are provided to perform visual inspection and evaluation of:

  1. Directional airflow
  2. Anterooms
  3. Primary containment systems
  4. Building ventilation system
  5. Heating, ventilation, and air-conditioning (HVAC) testing
  6. Filtration
  7. ABSL-3 and integration of individually ventilated caging, static caging systems, other elements; and downdraft tables
  8. Document validation
  9. Pressure reversal
  10. Failure testing
  11. Leakage issues related to HVAC
  12. Qualifications of testers

ANSI Z9.14 focuses on performance verification of ventilation system-engineering controls and related systems within a BSL-3/ABSL-3 laboratory.
Testing and verification of ventilation systems in BSL-3/ABSL-3 labs include:

  • Supply
  • Exhaust
  • Directional airflow
  • Biosafety cabinets
  • Air filtration
  • Exhaust stacks
  • Fan failure scenarios
  • Redundancy
  • Canopy hoods (autoclaves)
  • Specific ABSL-3 requirements

Testing and verification of related systems within BSL-3/ABSL-3 labs include:

  • Optimizing maintenance of pressure gradients and maintaining temperature and humidity
  • Physical integrity
  • Sealing and leakage factors
  • Interlocking systems
  • Airlocks
  • Doors/windows
  • Emergency and backup power systems
  • Alarms
  • Operating sequences
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